Na trama, o Flash Reverso usa a força de aceleração para voltar no tempo e afetar a vida do Flash. Batman, Superman, Mulher-Maravilha, Ciborgue, Zatanna e Senhor Destino são alguns dos personagens do filme.
The Flash / Barry Allen (voice)
Batman / Bruce Wayne (voice)
Superman / Clark Kent / Kal-El (voice) and Killer Croc / Waylon Jones (voice)
Reverse-Flash / Eobard Thawne (voice)
Wonder Woman / Diana Prince (voice) and Lois Lane (voice)
The Atom (voice)
Plastic Man / Patrick 'Eel' O'Brian (voice) and The Penguin / Oswald Cobblepot (voice)
Doctor Fate (voice) and Captain Cold / Leonard Snart (voice)
Cyborg / Victor Stone (voice)
Firestorm / Jason Rusch (voice)
Diretor